New Research Publication on Ovine Respiratory Syncytial Virus

I am pleased to announce that a paper on my research on the ovine strain of Respiratory Syncytial Virus was published in the October 2023 issue of the Canadian Journal of Veterinary Research. The paper describes the results from my recent abattoir survey of sheep pneumonia pathogens circulating in sheep flocks in southern Australia.

Pneumonia in sheep is common in Australia. As part of the abattoir survey, I collected bronchial swabs from 1095 sets of lungs representing 253 abattoir lots of sheep and lambs.

The bronchial swabs were tested for ovine Respiratory Syncytial Virus using an in-house qPCR based on the F gene sequence of the virus, with 2.4% testing positive.

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Joan Lloyd
Mycoplasma ovipneumoniae in Goats

Detection of Mycoplasma ovipneumoniae in goats is becoming increasingly common in Australia. So, what is Mycoplasma ovipneumoniae, how does is cause disease and what can goat owners do to find out if their goats are infected or, more importantly, prevent their goats becoming infected.

Mycoplasmas are a type of bacteria. Mycoplasma ovipneumoniae infects the lungs of goats.

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PCR to Identify Gastrointestinal Nematodes in Sheep and Goats

Parasitism with gastrointestinal nematodes (worms) is common in sheep and goats.

Most grazing sheep and goats are infected with several different types of gastrointestinal worms, with different pathogenic effects.

I have recently introduced PCR testing for two parasitic gastrointestinal nematodes of sheep and goats to my laboratory, including Haemonchus spp. (Barber's Pole worm) and Trichostrongylus spp. (Black Scour worm).

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Yersinia in Goats

Yersinia are a type of bacteria and are a significant cause of disease in goats.

Two species of Yersinia can cause disease in goats, Yersinia enterocolitica and Yersinia pseudotuberculosis.

Testing goats for Yersinia can be done as part of one of my on-farm small ruminant disease investigations.

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Real-Time PCR and Ct Values

Real-time PCR is a highly sensitive way to amplify and quantify a specific nucleic acid sequence in real time. In veterinary disease testing, the specific nucleic acid sequence is often from a pathogenic bacteria, virus, fungus, yeast, or parasite.

I am frequently asked what a Ct values means in a real-time PCR test result. I will try to explain what the Ct value means.

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PCR for Ovine Parainfluenza-3 Virus

Parainfluenza-3 virus is one of the most common viral infections of the respiratory tract of domestic ruminants. Historically it was thought that the bovine strain of parainfluenza-3 virus was responsible for infections and disease in sheep and goats. However, new molecular techniques indicate that sheep and goats may also have their own strains of the virus.

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